The facility uses two abi applied biosystems sequencers. Applied biosystems cycle sequencing kits available for dye terminator. Used applied biosystems 3xl genetic analyzer 2030217. Save target as to download a pdf version of the document to a destination. Explore the getting started and troubleshooting sections for solutions to top inquiries and common problems. Bid history for genetic analyzer, hitachi applied biosystems mdl 3xl hc1252. Gel electrophoresis is the movement of charged molecules through a gel solution such as polyacrylamide in an electrical field. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and realtime, digital and end point pcrfrom sample prep to data analysis. The applied biosystems 33xl genetic analyzer system includes both the instrument and the computeryou cannot start or restart one without starting the other. Applied biosystems instruments, assays, and reagents have been trusted in the lab for over 20 years. Applied biosystems 7500 getting started manual pdf. Applied biosystems 3730xl dna analyzer 1979b dna duration. You can also download our guide to dna sequence analysis. Rather than risking inconsistency as a result of pouring slabgels, a simple.
Samples are submitted in a halfskirted 96 well plate compatible with the 3xl sequencer. The applied biosystems 3100 is an automated, highthroughput, capillary electrophoresis system used for analyzing fluorescently labeled dna fragments find online auctions and classified ads for applied biosystems products on the labx marketplace. The purchase price of the applied biosystems 33xl genetic analyzer includes a grant of a limited, nontransferable license under u. Solid 5500xl sequencing by oligonucleotide ligation and detection developed by life technologies and made available in 2006, the solid 5500xl sequencer is the only next generation platform to sequence via primer ligation. Applied biosystems 3 genetic analyzer applied biosystems 3xl genetic analyzer. Applied biosystems 33xl genetic analyzers maintenance, troubleshooting, and reference guide v preface how to use this guide purpose of this guide the applied biosystems 33 xl genetic analyzers maintenance, troubleshooting, and reference guide provides instructions for maintaining the applied biosystems 33xl genetic analyzers. Price list for selected equipment, supplies and reagents. Applied biosystems 3 3xl genetic analyzers maintenance, troubleshooting, and reference guide v preface how to use this guide purpose of this guide the applied biosystems 33 xl genetic analyzers maintenance, troubleshooting, and reference guide provides instructions for maintaining the applied biosystems 3 3xl genetic analyzers. Dna sequence analysis bioinformatics genomics miami university. To speak with a representative, please fill out the contact form below or call us directly. Check out the most popular applied biosystems products for genetic analysis. Applied biosystems stretch 373a abi dna sequencer ebay. Across injections, the data indicates that high standard deviation values were obtained from the following loci. Upgrade of applied biosystems genetic analyzers, 3 series.
However, this wizard was dropped from the 3730xl and the 3xl sequencers. Abi 3xl dna sequencer the abi 3xl dna sequencer is a 16capillary instrument that offers highquality data, efficient sample processing, and 24hour unattended operation. Applied biosystems abi 3xl 16capillary array genetic. Improper boot sequence can lead to communication issues, aborted runs, and possible loss of data. Refurbished applied biosystems abi 3xl 16capillary array genetic analyzerdna sequencer analyzerdna sequencer pn 6280030 complete with new laser, windows xp pc, data collection 3. Within injections, the data shows no markers andor alleles with high standard deviation values exceeding 0. Seqtrace is designed for sequencing projects that require converting trace files directly into highquality. Core facility operates two applied biosystems 3xl sequencers. Applied biosystems 3 and 3xl dna analyzers key features 16 capillaries for the 3xl and. Applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. Dna sequencing by capillary electrophoresis by applied biosystems. Below are some of the key features and benefits of the abi 3xl. You can open and download a pdf using adobe acrobat.
Turn on the instrument by pressing the power button. The applied biosystems 3730xl dna analyzer can be used for dna fragment analysis applications such as microsatellites, aflp, snp analysis, mutation detection and traditional dna sequencing. Applied biosystems 33xl genetic analyzers getting started. It can process samples from 96 or 384 well plates, with mixed applications run on a single array. Increased sensitivity for amplified str alleles on. Once again on the applied biosystems 3xl sequencer service termination. Sequencing software applied biosystems sequence scanner. Capillary electrophoresis guides and catalog download. Applied biosystems 3730xl dna analyzer 1979b dna youtube. David fegredo medical scientistpathwestaustralia the abi 3 and 3xl genetic analyzers are the newest in a range of analyzers produced by abi. These machines use capillary electrophoresis, which is a significant advantage over slabgel based technologies. Abi 3xl sequencer available for sale at the lab world group. Before each run, the capillaries are automatically replenished with fresh electroosmotic flow eof suppression polymer.
Browse ads and contact the seller directly or request a quote for more details. To describe the standardized laboratory protocol for molecular subtyping of salmonella enterica serotype typhimurium. This increases lab productivity 4fold, without needing to buy another sequencer. Our facility is fully equipped for sanger dna sequencing, next generation. It came from a local lab that was upgrading equipment and we can accept pay pal or your credit card. The 3xl 16capillary array system is used to electrophoretically analyze samples following. The applied biosystems 3730 and 3730xl dna analyzer includes patented technology licensed from hitachi, ltd. Used applied biosystems 3xl genetic analyzer 2020339.
Similarly as a single capillary sequencer, abi prism 310 mentioned above, also 3 series of genetic analyzers abi3 and abi3xl, represent instruments that have outlived their expectations. Scope to provide pulsenet participants with a single protocol for performing mlva of s. Generaluberholter sequencer abi 3 abi 3xl vom profi zu verkaufen. Setupandanalysisusingyourowncustommlstscheme 1 introduction this tutorial illustrates the steps needed to set up your own mlst schema, e. Applied biosystems 33xl genetic analyzers getting started guide ix preface how to use this guide purpose of this guide the applied biosystems 33xl gene tic analyzers getting started guide provides stepbystep instructions for pr eparing and analyzing a sample. It has 12 configurable microfluidic flowchips with 6 independent lanes each to enable flexibility. Get compliance and validation services for applied biosystems and ion torrent instruments including nextgeneration and sanger sequencing platforms as well as their connected computers and electronic. Applied biosystems 3xl genetic analyzer get latest price a offering 16capillary throughput, advanced automation capabilities and superior performance, this system is ideal for mediumthroughput laboratories that need streamlined setup and 24hour unattended operation. Applied biosystems 33xl genetic analyzers maintenance, troubleshooting, and reference guide pn 4352716 contains information on maintaining the instrument and computer system, troubleshooting tables to diagnose performance failures, and advanced reference content. Applied biosystems 33xl genetic analyzers using data collection software v3. The sequencing is done on 3730xl with bigdye taq fs terminator v 3. Large and small sequencing projects are easily accommodated for internal and external customers. Once again on the applied biosystems 3xl sequencer service.
You may have noticed our june 2017 statement, where we informed you that. View, edit, print and export data generated using ab37030xl and other applied biosystems genetic. The applied biosystems 3 and 3xl genetic analyzers includes. Polymers can be used on the applied biosystems 3 and 3xl genetic analyzers as the separation matrix. The fully automated system can run hundreds of samples per day. Get compliance and validation services for applied biosystems and ion torrent instruments including nextgeneration and sanger sequencing platforms as. To learn more about current applications using sanger sequencing and to download the. Most importantly, as research throughput increases, the instrument can be upgraded to 16capillaries. To describe the standardized laboratory protocol for molecular subtyping of salmonella enterica serotype enteritidis. The abi prism 377 dna sequencer is an automated instrument designed for analyzing fluorescentlylabeled dna fragments by gel electrophoresis. Applied biosystems 310 genetic analyzer support thermo. Applied biosystems is one of the various brands under the life technologies brand of thermo fisher scientific corporation. Sequencing results are placed on a server for download from this web site to your.
Order applied biosystems user documents, msdss, certificates of analysis, and other related documents download pdf documents obtain information about customer training download software updates and patches applied biosystems 730075007500 fast realtime pcr system relative quantification getting started guide. Be sure to have these on hand when you are performing experiments with the 33xl genetic analyzers. The brand is focused on integrated systems for genetic analysis, which include computerized machines and the consumables used within them such as reagents in 2008, a merger between applied biosystems and invitrogen was finalized. The applied biosystems 5500xl solid sequencer provides embedded sequencing controls to maximize data quality and performance. Designed for mediumthroughput applications, it is capable of sequencing and fragment analysis including microsatellite, aflp, loh, and snp validation and screening. Abi prism dna sequencing analysis or abi prism genescan analysis software. This applied biosystems stretch 373a dna sequencer is in real good condition and guaranteed to functions 100%. Applied biosystems 3 genetic analyzer thermo fisher. Wait until the desktop appears and all programs have finished loading hard drive light on computer stops flashing. Applied biosystems 3 3xl genetic analyzers support. Level 1 dna sequencing on applied biosystems 3xl genetic analyzer. For more information regarding licenses, please contact the director of licensing at applied biosystems, 850 lincoln centre drive, foster city, california 94404, usa. Applied biosystems 3 3xl genetic analyzers getting started guide ix preface how to use this guide purpose of this guide the applied biosystems 3 3xl gene tic analyzers getting started guide provides stepbystep instructions for pr eparing and analyzing a sample. Applied biosystems 33xl genetic analyzers supporttroubleshooting applied biosystems 37303730xl dna analyzers support applied biosystems 35003500xl genetic analyzers support applied biosystems seqstudio genetic analyzer support.
The genotyping and fragment sizing are done on 3xl please consult our recommendations for template preparation to avoid failures. Pcr products were analysed on an abi prism 3 xl genetic analyzer applied biosystems with a 50 cm capillary and pop7 applied biosystems. Applied biosystems 3 and 3xl dna analyzers key features 16 capillaries for the 3 xl and 4 capillaries for the 3 system 24hour unattended operation easy instrument setup. Page 1 applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. Applied biosystems 37303730 xl our scientific research. Browse through the guides and tools section to access comprehensive productrelated support resources. The applied biosystems 3xl is a 16capillary electrophoresis system for genetic analysis. Applied biosystems 3xl is just one of the fantastic products created by a company who has a long standing tradition of providing excellence in life science instruments, reagents and software find online auctions and classified ads for applied biosystems products on the labx marketplace. The abi 3 is another ideal dna sequencer for low to midthroughput labs. Manufacturer of applied biosystems 3 genetic analyzer. System is complete with computer and original disks.
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